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[下载]Applied Electrospray Mass Spectrometry mass spectrometry最新收录

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[下载]Applied Electrospray Mass Spectrometry mass spectrometry最新收录Introduction:In the last decade, electrospray ionization mass spectrometry (ESI–MS) has become the most universally applied mass spectrometric technique for nonvolatile, thermal-labile molecules ranging in size from small compounds(300–1000 Da) to large biomolecules with molecular weights in excess of 150kDa. Electrospray ionization emerged from the pioneering experiments of J.Zeleny (1917) and later those of Malcolm Dole (late 1960s), who performed aseries of experiments defining the parameters required for introducing large molecules as ions into the gas phase of a mass spectrometer. In 1984, John Fenn and his coworker M. Yamashita in the United States and M. L.Aleksandrov and his coworkers in the U.S.S.R. first reported the successful coupling of ESI with quadrupole and magnetic sector mass analyzers, respectively.Because ESI is relatively simple and works at atmospheric pressure, it can be easily coupled to both high-performance liquid chromatography, allowing the analysis of complex mixtures, and devices for automated sample analysis— satisfying the quest among mass spectrometrists to provide an interface to liquid separation methods. From the beginning, ESI showed signs that it would become the primary MS tool in pharmaceutical and biomedical research, especially in high-throughput screening of large chemical libraries,impurity analysis, pharmacokinetics and metabolite identification, and protein characterization.This book brings together the work of contributors from academia and industry who have played important roles in the continuing development of this technique. Our goal is to present a compilation of the latest applications of ESI to practitioners of mass spectrometry, as well as to other scientists interested in applying this technique to their areas of research. The principal theme of the book is mass spectrometric analysis in the areas of pharmaceutical, peptide,and protein chemistry.The book begins with a historical review of the ESI technique — its principles and instrumentation (Chapter 1), followed by a description of nanospray development and applications in macromolecules (Chapter 2). The remaining chapters cover applications of small molecules (Chapters 3, 4, and 5), peptides/proteins (Chapters 6, 7, and 8), noncovalent complexes (Chapter 9), special topics in protein folding/unfolding (Chapter 10), and clinical/biomedicalapplications (Chapter 11). Topics such as the structural elucidation of complex natural products (Chapter 3), analysis of libraries of compounds prepared using combinatorial chemistry (Chapter 4), and the application of ESI-MS in the field of pharmacokinetics and drug metabolism studies (Chapter 5) are of great importance to scientists in the pharmaceutical industries and aredescribed in great detail. Chapters 6, 7, and 8 explore the critical role of the ESI technique in structural characterization of peptides/proteins, especially in the rapidly evolving area of proteomics and in identifying gene-expressed proteins in a high-throughput process (Chapter 8). Dynamic structures of proteins, such as noncovalent interactions and folding/unfolding, are further covered in Chapters 9 and 10. Finally, Chapter 11 deals with the application of microflowESI–MS in problems in the field of clinical and biomedical research.We acknowledge the special efforts of all the authors, who have made great contributions to this book. We also thank the Schering-Plough Research Institute, Washington University, and the National Center for Research Resources of the National Institutes of Health for their support. Special thanks go to the acquisitions and production editors at Marcel Dekker, Inc., for their assistance. Birendra N. PramanikA. K. Ganguly Michael L. Gross
 
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